Journal: International Journal of Molecular Sciences

Article Title: High-Fat Diet Consumption in Adolescence Induces Emotional Behavior Alterations and Hippocampal Neurogenesis Deficits Accompanied by Excessive Microglial Activation

doi: 10.3390/ijms23158316

Figure Lengend Snippet: Effect of HFD consumption in adolescence on DCX and Ki67 expression in the mouse hippocampus. ( A ) Fluorescence staining of DCX + and Ki67 + cells in the dorsal and ventral hippocampus of CD- and HFD-fed mice. Green, red and blue fluorescence represent Ki67, DCX, and DAPI, respectively. The numbers of Ki67 + and DCX + cells in the dSGZ ( B , C ) and vSGZ ( D , E ) were counted. The data are expressed as the mean ± SEM ( n = 8 mice per group). When the HFD group was compared with the CD group, the data were analyzed using Student’s t -test. ns, not significant; * p < 0.05.

Article Snippet: The following antibodies were used: rabbit anti-Ki67 (for proliferating cells, 1:500, ab16667, Abcam, Cambridge, UK), guinea pig anti-DCX (for newly generated neurons, 1:1000, AB2253, Millipore, Temecula, CA, USA), rabbit anti-Iba1 (for microglia, 1:1000, 019-19741, Wako, Osaka, Japan), rat anti-CD68 (for phagocytic microglia, 1:1500, MCA1957, Bio-Rad, Oxford, UK), goat anti-Iba1 (for microglia, 1:600, 011-27991, Wako), Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:1000, ab150077, Abcam), Alexa Fluor 568-conjugated goat anti-guinea pig IgG (1:1000, ab175714, Abcam), Alexa Fluor 594-conjugated donkey anti-rat IgG (1:1000, ab150156, Abcam), and Alexa Fluor 488-conjugated donkey anti-goat IgG (1:1000, ab150129, Abcam).

Techniques: Expressing, Fluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: High-Fat Diet Consumption in Adolescence Induces Emotional Behavior Alterations and Hippocampal Neurogenesis Deficits Accompanied by Excessive Microglial Activation

doi: 10.3390/ijms23158316

Figure Lengend Snippet: Linear regression analysis of hippocampal neurogenesis and depression-like and anxiety-like behavior. ( A , B ) Correlation between the density of Ki67 + cells in the dSGZ/vSGZ and sucrose preference in the SPT. ( C ) Correlation between the density of DCX + cells in the vSGZ and sucrose preference in the SPT. ( D ) Correlation between the dendrite length of DCX + cells in the vDG and sucrose preference in the SPT. ( E , F ) Correlation between the density of Ki67 + cells in the dSGZ/vSGZ and the time spent in the central area in the OFT. ( G ) Correlation between the density of DCX + cells in the vSGZ and time spent in the central area in the OFT. ( H ) Correlation between the dendrite length of DCX + cells in the vDG and the time spent in the central area in the OFT. Mice from both the CD group (represented as gray triangles) and HFD group (represented as orange triangles) are included ( n = 6–8 mice per group). The solid black lines, gray dotted lines, and orange dotted lines indicate linear regression for mice fed a CD or HFD, mice fed a CD, and mice fed an HFD, respectively. * means statistical significance after Bonferroni correction.

Article Snippet: The following antibodies were used: rabbit anti-Ki67 (for proliferating cells, 1:500, ab16667, Abcam, Cambridge, UK), guinea pig anti-DCX (for newly generated neurons, 1:1000, AB2253, Millipore, Temecula, CA, USA), rabbit anti-Iba1 (for microglia, 1:1000, 019-19741, Wako, Osaka, Japan), rat anti-CD68 (for phagocytic microglia, 1:1500, MCA1957, Bio-Rad, Oxford, UK), goat anti-Iba1 (for microglia, 1:600, 011-27991, Wako), Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:1000, ab150077, Abcam), Alexa Fluor 568-conjugated goat anti-guinea pig IgG (1:1000, ab175714, Abcam), Alexa Fluor 594-conjugated donkey anti-rat IgG (1:1000, ab150156, Abcam), and Alexa Fluor 488-conjugated donkey anti-goat IgG (1:1000, ab150129, Abcam).

Techniques: